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  • Caffeine 20-second quick test-GTC300

    Developed in cooperation with BAKE Tech., the patent rights belong to BAKE Tech.

    No consumables required, can detect caffeine and chlorogenic acid

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    Caffeine/Chlorogenic acid ratio determines much of the flavor of coffee.

    Caffeine and chlorogenic acid are the two main components in coffee, and they have an important impact on the flavor of coffee.

     

    Caffeine: Caffeine gives coffee a bitter taste. If the caffeine content in coffee is high, it may make the coffee taste more bitter.

     

    Chlorogenic Acids: It gives coffee a sour taste. This sour flavor can come in many variations, including bright, mellow or fruity. Coffee beans grown at high altitudes typically contain higher concentrations of chlorogenic acid, which adds to the variety of coffee flavors.

  • GTC300 can detect caffeine/chlorogenic acid, and you can train yourself to brew coffee every time to verify the stability.

     You can also test whether the coffee flavor is the same among peers.

    Factors such as coffee powder particle size, water temperature and brewing technique can affect the extraction rate of caffeine and chlorogenic acid, thereby affecting the flavor of coffee. However, everyone has different feelings about the taste, so multiple people need to taste it and then average the taste results to increase fairness. GTC300 uses a scientific method to measure and calculate the caffeine and chlorogenic acid content, giving you the tools to judge the flavor of coffee. .

     

    The reproducibility of brewing coffee every time is because each person's taste and taste perception are unique and are affected by taste bud sensitivity, physiological differences, differences in experience and memory, which will produce different feelings.

     

    A good tool for you to understand coffee. Scientifically measuring the caffeine and chlorogenic acid content can help you more objectively, in addition to training yourself on the stability of brewing coffee.

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  • Reproducibility test of GTC300

    This is a rapid screening equipment that uses spectral absorption as the design principle. Since the consumable material is clean paper towels, please measure at least 3 times and average it.

     

    First-time users should use it more often to familiarize themselves with the feel and try to keep the quartz chips free of bubbles.

     

    Operate GTC300 and test 11 times. In the 9th time, the data was too low, so it was eliminated. Of course, you can also measure 3 times and average it.

     

    When operating instruments, attention should be paid to data reproducibility. Only when data is reproducible can it have reference value.

  • Measurement technology is built on the spectral architecture

     GTC300 shrinks the spectrometer architecture into a machine.

     

    The spectrometer has a light source, the light is transmitted through the optical fiber, the light penetrates the cuvette, the liquid to be measured is in the cuvette, and the degree of light absorption of the liquid to be measured is judged.

     

    The absorbance level is detected using a spectrometer, and the results are read through a computer to obtain the absorption spectrum of the object to be measured. The concentration is then determined from the absorption spectrum.

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    GTC300 reduces the structure of the spectrometer and uses aluminum fixtures to fix the light source and sensor.

     

    A fixed light path is created between the aluminum fixtures, and there is a detection hole between the light paths. A quartz plate is placed in the inspection hole. The liquid to be measured is between the quartz plate and the quartz plate. The quartz plate is like a cuvette.

     

    The microprocessor is used for signal judgment and calculation.

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  • How to calibrate GTC300

    A laboratory certified by ISO/IEC 170 was entrusted to use HPLC to measure caffeine/chlorogenic acid in coffee and caffeine in tea. This liquid was used as a standard.

     

    Use standard solution to calibrate the GTC300, and because the PCB circuits cannot be exactly the same, the GTC300 must be calibrated one by one.

  • GTC300 usage restrictions

    This is the spectrum after diluting coffee 30 times. Because of direct measurement, the deuterium light source cannot penetrate the coffee. If it cannot penetrate, it cannot be analyzed, so it must be diluted.

     

    GTC300 uses quartz chips to hold the coffee. This action is like diluting, so the user can directly measure it after dripping.

     

    GTC300 uses 4 light sources, 280nm for caffeine, 320nm for chlorogenic acid, and the remaining 2 are used for calibration.

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  • GTC300 mainly uses the measurement method of spectrometer as the judgment principle. It is a rapid screening device. Because it is based on the principle of spectrometer, it has some limitations, which are explained as follows:

    1. It is impossible to measure when the temperature of the liquid to be measured is too high:

    Because molecules and atoms in liquids move and vibrate at different speeds at different temperatures, this phenomenon will cause signal differences.

    Therefore, it is not recommended to measure at high temperatures. You can put it into a small cup and return it to room temperature, or use room temperature drinking water to dilute the liquid to be tested, and multiply the dilution factor after the measurement.

  • 2. For low-caffeine coffee, additional standard lines must be established:

    If you have low-caffeine measurement needs, we can help you create low-caffeine caffeine and chlorogenic acid judgment options.
     

    GTC300 can quickly screen caffeine and chlorogenic acid in coffee brewed on site.

     

    This is a spectrum chart. It can be clearly seen from the chart that there is a big difference in the spectrum of caffeine/chlorogenic acid at different concentrations, which can be easily judged by GTC300.

     

    But the spectrum of decaffeinated coffee is similar to the spectrum of lower concentration coffee.

     

    If low-caffeine measurement is used, the data will be the same as that of low-concentration coffee. Although the caffeine absorption spectrum of low-caffeine drifts to 290nm, the half-wave width of the LED is about 15~20nm, so it is impossible to judge low-caffeine drinks. .

     

    However, the low caffeine standard curve can be set independently.

     

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  • 3. Measure caffeine linearity, currently set within 120mg/100mL:
    If the measurement data shows 0 mg/100mL and out of range appears on the screen, please dilute the solution to be tested.

    Set within 120mg/100mL to increase stability.

    Each sensor has an output voltage, and the output voltage corresponds to the measured caffeine concentration.

     

    Although the measurement range can be very wide, the operation of rapid screening equipment is not like laboratory instruments. Impurities must be removed and the operation must be precise, so the signal will jump. When the signal jumps, it corresponds to the detection result.

     

    As can be seen in the figure, the performance of output voltage and concentration, so GTC300 deliberately sets the measurement range to within 120mg/100mL.

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  • 4. For high concentration coffee, please dilute it:

    When the liquid being measured is high-concentration coffee, such as Espresso, it is recommended to dilute it at least 6 times.

     

    The video explains that the measurement data of high-concentration coffee is 0 mg/100mL, which means that the light source cannot penetrate the coffee, so it needs to be diluted. After dilution, the measurement result is multiplied by the dilution factor.

     

    Please prepare tips, drinking water and centrifuge tubes first.

    Dilute 6 times: 500 uL drinking water, 100 uL Espresso, then use a micropipette to stir and measure after stirring.

  • Automatically display results

    After detection by GTC300, caffeine and chlorogenic acid will be automatically displayed on the touch screen, and the total volume of coffee can be entered, and the total amount of caffeine will be automatically calculated for your reference.

    Specification

    Applications: Caffeine/Chlorogenic Acid Detection

    Range:

    Caffeine: 5~120mg/100mL;

    Chlorogenic acid: 5~120mg/100mL

    Accuracy: 5%

    Reproducibility: 8%

    Operating temperature: 20– 35 °C

    Measurement time: 20 s

    Power supply: DC 5V 2A

    Accessories: charger, charging cable, quantitative pipette, 2mL centrifuge tube (diluted coffee) and outer box

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    Q and A

    Can coffee concentration (TDS) be used to determinecaffeine?

    Although both coffee concentration (TDS)and water quality tester (TDS) are defined as Total Dissolved Solids, the water
    quality tester measures the conductivity of ions in pure water, while the coffee concentration (TDS) measures not only the conductivity of coffee, but also the refractive index (Diopter) of the liquid, since Coffeeconsists mainly of caffeine, chlorogenic acid, tannins, fixed oils,
    carbohydrates, and proteins to determine the degree of extraction, and the TDS measures the turbidity of the coffee liquid. Simply put, the result of TDS is a measure of the cloudiness of the liquid, the lower TDS values indicate clearer
    water, while higher TDS values indicate cloudier water with more impurities.

    Are there any conditions for the 20-Seconds Caffeine QuickDetection?

    Yes, only beverages preparedon-site can be detected by 20-Seconds Caffeine Quick Detection.

    Canned beverages may contain milk, soda,or other flavorings that can interfere with the accuracy of the detection. Therefore,
    please use freshly prepared coffee, tea, or cocoa for the test.

    Why is it possible todetect caffeine concentration in 20 Seconds without pretreatment?

    For dropping coffee on the quartz plateitself is as a dilution pretreatment. Due to the diverse content of substancesin coffee, the color of coffee appears in brown and the light signal cannot penetrate directly, making it difficult to analyze. Therefore, by diluting it at least 30 times , characteristic peaks of caffeine can be obtained. By using a powerful magnet to control theforce holding the testing sample, and using quartz to hold the liquid on the side, the amount of testing sample used is only 0.1CC. This process is similar to dilution, which can eliminate the influence of color and other interferences, allowing light to directly penetrate and to be analyzed. This is the technology behind the 20-Seconds Quick Caffeine Detection.

    How to use the Caffeine 20-SecondsQuick Detector accurately?

    Please take multiplemeasurements and calculate the average.

    To increase accuracy, it is recommendedto perform multiple measurements and take the average. Following the HPCL (High
    Performance Liquid Chromatography) judging method, each bottle of sample should be tested three times and the average of the three measurements should be taken. As the 20-Seconds Caffeine Rapid Detection uses optical absorption method, if one of the three measurements is significantly higher than the other two, it should be excluded, and the measurement should be repeated.

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